Efficient Genome Editing in Clostridium cellulolyticum via CRISPR-Cas9 Nickase

The CRISPR-Cas9 system is a powerful and revolutionary genome-editing tool for eukaryotic genomes, but its use in bacterial genomes is very limited. Here, we investigated the use of the Streptococcus pyogenes CRISPR-Cas9 system in editing the genome of Clostridium cellulolyticum, a model microorgani...

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Detalles Bibliográficos
Publicado en:Appl Environ Microbiol
Autores principales: Xu, Tao, Li, Yongchao, Shi, Zhou, Hemme, Christopher L., Li, Yuan, Zhu, Yonghua, Van Nostrand, Joy D., He, Zhili, Zhou, Jizhong
Formato: Artigo
Lenguaje:Inglês
Publicado: American Society for Microbiology 2015
Materias:
Methods
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC4475897/
https://ncbi.nlm.nih.gov/pubmed/25911483
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.00873-15
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