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Efficient Genome Editing in Clostridium cellulolyticum via CRISPR-Cas9 Nickase
The CRISPR-Cas9 system is a powerful and revolutionary genome-editing tool for eukaryotic genomes, but its use in bacterial genomes is very limited. Here, we investigated the use of the Streptococcus pyogenes CRISPR-Cas9 system in editing the genome of Clostridium cellulolyticum, a model microorgani...
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| Publicado no: | Appl Environ Microbiol |
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| Main Authors: | , , , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Microbiology
2015
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4475897/ https://ncbi.nlm.nih.gov/pubmed/25911483 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.00873-15 |
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