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Development of an Efficient Genome Editing Tool in Bacillus licheniformis Using CRISPR-Cas9 Nickase

Bacillus strains are important industrial bacteria that can produce various biochemical products. However, low transformation efficiencies and a lack of effective genome editing tools have hindered its widespread application. Recently, clustered regularly interspaced short palindromic repeat (CRISPR...

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Detalhes bibliográficos
Publicado no:Appl Environ Microbiol
Main Authors: Li, Kaifeng, Cai, Dongbo, Wang, Zhangqian, He, Zhili, Chen, Shouwen
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5835740/
https://ncbi.nlm.nih.gov/pubmed/29330178
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.02608-17
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