SWISS: multiplexed orthogonal genome editing in plants with a Cas9 nickase and engineered CRISPR RNA scaffolds

We describe here a CRISPR simultaneous and wide-editing induced by a single system (SWISS), in which RNA aptamers engineered in crRNA scaffold recruit their cognate binding proteins fused with cytidine deaminase and adenosine deaminase to Cas9 nickase target sites to generate multiplexed base editin...

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Salvato in:
Dettagli Bibliografici
Pubblicato in:Genome Biol
Autori principali: Li, Chao, Zong, Yuan, Jin, Shuai, Zhu, Haocheng, Lin, Dexing, Li, Shengnan, Qiu, Jin-Long, Wang, Yanpeng, Gao, Caixia
Natura: Artigo
Lingua:Inglês
Pubblicazione: BioMed Central 2020
Soggetti:
Method
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC7296638/
https://ncbi.nlm.nih.gov/pubmed/32546280
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13059-020-02051-x
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