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Multigene Editing in the Escherichia coli Genome via the CRISPR-Cas9 System
An efficient genome-scale editing tool is required for construction of industrially useful microbes. We describe a targeted, continual multigene editing strategy that was applied to the Escherichia coli genome by using the Streptococcus pyogenes type II CRISPR-Cas9 system to realize a variety of pre...
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| Publié dans: | Appl Environ Microbiol |
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| Auteurs principaux: | , , , , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
American Society for Microbiology
2015
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4357945/ https://ncbi.nlm.nih.gov/pubmed/25636838 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.04023-14 |
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