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High‐Throughput Kinetic Analysis for Target‐Directed Covalent Ligand Discovery

Cysteine‐reactive small molecules are used as chemical probes of biological systems and as medicines. Identifying high‐quality covalent ligands requires comprehensive kinetic analysis to distinguish selective binders from pan‐reactive compounds. Quantitative irreversible tethering (qIT), a general m...

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Dettagli Bibliografici
Pubblicato in:Angew Chem Int Ed Engl
Autori principali: Craven, Gregory B., Affron, Dominic P., Allen, Charlotte E., Matthies, Stefan, Greener, Joe G., Morgan, Rhodri M. L., Tate, Edward W., Armstrong, Alan, Mann, David J.
Natura: Artigo
Lingua:Inglês
Pubblicazione: John Wiley and Sons Inc. 2018
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC5947712/
https://ncbi.nlm.nih.gov/pubmed/29480525
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/anie.201711825
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