Orthologous CRISPR-Cas9 enzymes for Combinatorial Genetic Screens

Combinatorial genetic screening using CRISPR-Cas9 is a useful approach to uncover redundant genes and to explore complex gene networks. However, current approaches suffer from interference between the single-guide RNAs (sgRNAs) and from limited gene targeting activity. To increase the efficiency of...

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Bibliographic Details
Published in:Nat Biotechnol
Main Authors: Najm, Fadi J, Strand, Christine, Donovan, Katherine F, Hegde, Mudra, Sanson, Kendall R, Vaimberg, Emma W, Sullender, Meagan E, Hartenian, Ella, Kalani, Zohra, Fusi, Nicolo, Listgarten, Jennifer, Younger, Scott T, Bernstein, Bradley E, Root, David E, Doench, John G
Format: Artigo
Language:Inglês
Published: 2017
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Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC5800952/
https://ncbi.nlm.nih.gov/pubmed/29251726
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nbt.4048
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