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Real-time fluorescence assays to monitor duplex unwinding and ATPase activities of helicase proteins
Many physiological functions of helicases are dependent on their ability to unwind nucleic acid duplexes in an ATP-dependent fashion. Determining the kinetic frameworks of these processes is critical to understand how these proteins function. We recently developed a fluorescence assay to monitor RNA...
Gorde:
| Argitaratua izan da: | Nat Protoc |
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| Egile Nagusiak: | , , , , |
| Formatua: | Artigo |
| Hizkuntza: | Inglês |
| Argitaratua: |
2014
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| Gaiak: | |
| Sarrera elektronikoa: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5524973/ https://ncbi.nlm.nih.gov/pubmed/24945382 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2014.112 |
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