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A Graphene‐Based Platform for the Assay of Duplex‐DNA Unwinding by Helicase
Time to unwind: Graphene oxide (GO) enables the quantitative measurement of helicase‐dependent double‐stranded DNA (dsDNA) unwinding activity in real time. GO selectively binds to unwound fluorescent‐dye‐labeled single‐stranded DNA and quenches its fluorescence (see picture). The helicase activity i...
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| Publié dans: | Angew Chem Int Ed Engl |
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| Auteurs principaux: | , , , , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
WILEY‐VCH Verlag
2010
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7159720/ https://ncbi.nlm.nih.gov/pubmed/20818755 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/anie.201001332 |
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