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A Graphene‐Based Platform for the Assay of Duplex‐DNA Unwinding by Helicase
Time to unwind: Graphene oxide (GO) enables the quantitative measurement of helicase‐dependent double‐stranded DNA (dsDNA) unwinding activity in real time. GO selectively binds to unwound fluorescent‐dye‐labeled single‐stranded DNA and quenches its fluorescence (see picture). The helicase activity i...
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| Udgivet i: | Angew Chem Int Ed Engl |
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| Main Authors: | , , , , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
WILEY‐VCH Verlag
2010
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7159720/ https://ncbi.nlm.nih.gov/pubmed/20818755 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/anie.201001332 |
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