Efficient method for site-directed mutagenesis in large plasmids without subcloning

Commonly used methods for site-directed DNA mutagenesis require copying the entire target plasmid. These methods allow relatively easy modification of DNA sequences in small plasmids but become less efficient and faithful for large plasmids, necessitating full sequence verification. Introduction of...

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Detaylı Bibliyografya
Yayımlandı:PLoS One
Asıl Yazarlar: Hallak, Louay K., Berger, Kelly, Kaspar, Rita, Kwilas, Anna R., Montanaro, Federica, Peeples, Mark E.
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: Public Library of Science 2017
Konular:
Research Article
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC5456045/
https://ncbi.nlm.nih.gov/pubmed/28575024
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0177788
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