High-efficiency oligonucleotide-directed plasmid mutagenesis.
A number of single- and double-base substitutions have been introduced into either the polylinker region or the lacZ gene in the plasmid vector pUC19. The efficiencies of these changes upon transfection of TG-1 bacterial cells were generally 70-80%. A strategy has been devised by which the wild-type...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Principais autores: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1990
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC53493/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2154748/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.87.4.1451 |
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