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High-efficiency oligonucleotide-directed plasmid mutagenesis.

A number of single- and double-base substitutions have been introduced into either the polylinker region or the lacZ gene in the plasmid vector pUC19. The efficiencies of these changes upon transfection of TG-1 bacterial cells were generally 70-80%. A strategy has been devised by which the wild-type...

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Hlavní autoři: Olsen, D B, Eckstein, F
Médium: Artigo
Jazyk:Inglês
Vydáno: 1990
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC53493/
https://ncbi.nlm.nih.gov/pubmed/2154748
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