Laddar...
Efficient Site-Directed Saturation Mutagenesis Using Degenerate Oligonucleotides
We describe a reliable protocol for constructing single-site saturation mutagenesis libraries consisting of all 20 naturally occurring amino acids at a specific site within a protein. Such libraries are useful for structure-function studies and directed evolution. This protocol extends the utility o...
Sparad:
| Huvudupphovsmän: | , |
|---|---|
| Materialtyp: | Artigo |
| Språk: | Inglês |
| Publicerad: |
The Association of Biomolecular Resource Facilities
2007
|
| Ämnen: | |
| Länkar: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2062545/ https://ncbi.nlm.nih.gov/pubmed/17595310 |
| Taggar: |
Lägg till en tagg
Inga taggar, Lägg till första taggen!
|