Efficient Site-Directed Saturation Mutagenesis Using Degenerate Oligonucleotides

We describe a reliable protocol for constructing single-site saturation mutagenesis libraries consisting of all 20 naturally occurring amino acids at a specific site within a protein. Such libraries are useful for structure-function studies and directed evolution. This protocol extends the utility o...

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Bibliographic Details
Main Authors: Steffens, David L., Williams, John G. K.
Format: Artigo
Language:Inglês
Published: The Association of Biomolecular Resource Facilities 2007
Subjects:
Communication
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2062545/
https://ncbi.nlm.nih.gov/pubmed/17595310
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