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Efficient Site-Directed Saturation Mutagenesis Using Degenerate Oligonucleotides
We describe a reliable protocol for constructing single-site saturation mutagenesis libraries consisting of all 20 naturally occurring amino acids at a specific site within a protein. Such libraries are useful for structure-function studies and directed evolution. This protocol extends the utility o...
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| Main Authors: | , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
The Association of Biomolecular Resource Facilities
2007
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2062545/ https://ncbi.nlm.nih.gov/pubmed/17595310 |
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