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Improved oligonucleotide site-directed mutagenesis using M13 vectors.
An improved method is described for the construction of mutations in M13 vectors using synthetic oligonucleotides. The DNA is first cloned into a novel M13 vector (based upon M13mp18 or M13mp19), which carries a genetic marker that can be selected against, such as an EcoK or EcoB site, or an amber m...
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| Main Authors: | , , |
|---|---|
| Formáid: | Artigo |
| Teanga: | Inglês |
| Foilsithe: |
1985
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| Rochtain Ar Líne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC321798/ https://ncbi.nlm.nih.gov/pubmed/2989795 |
| Clibeanna: |
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