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Improved oligonucleotide site-directed mutagenesis using M13 vectors.

An improved method is described for the construction of mutations in M13 vectors using synthetic oligonucleotides. The DNA is first cloned into a novel M13 vector (based upon M13mp18 or M13mp19), which carries a genetic marker that can be selected against, such as an EcoK or EcoB site, or an amber m...

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Hlavní autoři: Carter, P, Bedouelle, H, Winter, G
Médium: Artigo
Jazyk:Inglês
Vydáno: 1985
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC321798/
https://ncbi.nlm.nih.gov/pubmed/2989795
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