In vitro site-directed mutagenesis with synthetic DNA oligonucleotides yields unexpected deletions and insertions at high frequency.

We have used in vitro site-directed mutagenesis with synthetic DNA oligonucleotides to introduce single nucleotide mutations in yeast mtDNA. In addition to the expected DNA alterations we also recovered with high frequency mutants with large deletions and insertions which arose through interaction w...

ver descrição completa

Detalhes bibliográficos

Publicado no:	Nucleic Acids Res
Principais autores:	Osinga, K A, Van der Bliek, A M, Van der Horst, G, Groot Koerkamp, M J, Tabak, H F, Veeneman, G H, Van Boom, J H
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Oxford University Press 1983
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC326610/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6324090/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/11.24.8595
Tags:	Adicionar Tag Sem tags, seja o primeiro a adicionar uma tag!