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In vitro site-directed mutagenesis with synthetic DNA oligonucleotides yields unexpected deletions and insertions at high frequency.

We have used in vitro site-directed mutagenesis with synthetic DNA oligonucleotides to introduce single nucleotide mutations in yeast mtDNA. In addition to the expected DNA alterations we also recovered with high frequency mutants with large deletions and insertions which arose through interaction w...

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Main Authors: Osinga, K A, Van der Bliek, A M, Van der Horst, G, Groot Koerkamp, M J, Tabak, H F, Veeneman, G H, Van Boom, J H
格式: Artigo
語言:Inglês
出版: 1983
在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC326610/
https://ncbi.nlm.nih.gov/pubmed/6324090
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