In vitro site-directed mutagenesis with synthetic DNA oligonucleotides yields unexpected deletions and insertions at high frequency.

We have used in vitro site-directed mutagenesis with synthetic DNA oligonucleotides to introduce single nucleotide mutations in yeast mtDNA. In addition to the expected DNA alterations we also recovered with high frequency mutants with large deletions and insertions which arose through interaction w...

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Bibliografische gegevens

Gepubliceerd in:	Nucleic Acids Res
Hoofdauteurs:	Osinga, K A, Van der Bliek, A M, Van der Horst, G, Groot Koerkamp, M J, Tabak, H F, Veeneman, G H, Van Boom, J H
Formaat:	Artigo
Taal:	Inglês
Gepubliceerd in:	Oxford University Press 1983
Online toegang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC326610/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6324090/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/11.24.8595
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