Transformation of yeast with synthetic oligonucleotides.

Genomic DNA of the yeast, Saccharomyces cerevisiae, can be conveniently and specifically altered by transforming spheroplasts or lithium acetate-treated cells directly with synthetic oligonucleotides. Altered forms of iso-1-cytochrome c were generated by transforming a cyc1 mutant with oligonucleoti...

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Detalhes bibliográficos

Publicado no:	Proc Natl Acad Sci U S A
Principais autores:	Moerschell, R P, Tsunasawa, S, Sherman, F
Formato:	Artigo
Idioma:	Inglês
Publicado em:	National Academy of Sciences 1988
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC279583/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2829192/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.85.2.524
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