Transformation of yeast with synthetic oligonucleotides.
Genomic DNA of the yeast, Saccharomyces cerevisiae, can be conveniently and specifically altered by transforming spheroplasts or lithium acetate-treated cells directly with synthetic oligonucleotides. Altered forms of iso-1-cytochrome c were generated by transforming a cyc1 mutant with oligonucleoti...
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| Publicat a: | Proc Natl Acad Sci U S A |
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| Autors principals: | , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
National Academy of Sciences
1988
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC279583/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2829192/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.85.2.524 |
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