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In vitro site-directed mutagenesis with synthetic DNA oligonucleotides yields unexpected deletions and insertions at high frequency.

We have used in vitro site-directed mutagenesis with synthetic DNA oligonucleotides to introduce single nucleotide mutations in yeast mtDNA. In addition to the expected DNA alterations we also recovered with high frequency mutants with large deletions and insertions which arose through interaction w...

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Detalhes bibliográficos
Main Authors: Osinga, K A, Van der Bliek, A M, Van der Horst, G, Groot Koerkamp, M J, Tabak, H F, Veeneman, G H, Van Boom, J H
Formato: Artigo
Idioma:Inglês
Publicado em: 1983
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC326610/
https://ncbi.nlm.nih.gov/pubmed/6324090
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