In vitro site-directed mutagenesis with synthetic DNA oligonucleotides yields unexpected deletions and insertions at high frequency.

We have used in vitro site-directed mutagenesis with synthetic DNA oligonucleotides to introduce single nucleotide mutations in yeast mtDNA. In addition to the expected DNA alterations we also recovered with high frequency mutants with large deletions and insertions which arose through interaction w...

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Detalles Bibliográficos

Publicado en:	Nucleic Acids Res
Principais autores:	Osinga, K A, Van der Bliek, A M, Van der Horst, G, Groot Koerkamp, M J, Tabak, H F, Veeneman, G H, Van Boom, J H
Formato:	Artigo
Idioma:	Inglês
Publicado:	Oxford University Press 1983
Acceso en liña:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC326610/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6324090/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/11.24.8595
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