In vitro site-directed mutagenesis with synthetic DNA oligonucleotides yields unexpected deletions and insertions at high frequency.

We have used in vitro site-directed mutagenesis with synthetic DNA oligonucleotides to introduce single nucleotide mutations in yeast mtDNA. In addition to the expected DNA alterations we also recovered with high frequency mutants with large deletions and insertions which arose through interaction w...

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Bibliografski detalji

Izdano u:	Nucleic Acids Res
Glavni autori:	Osinga, K A, Van der Bliek, A M, Van der Horst, G, Groot Koerkamp, M J, Tabak, H F, Veeneman, G H, Van Boom, J H
Format:	Artigo
Jezik:	Inglês
Izdano:	Oxford University Press 1983
Online pristup:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC326610/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6324090/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/11.24.8595
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