Chargement en cours...
Improved oligonucleotide site-directed mutagenesis using M13 vectors.
An improved method is described for the construction of mutations in M13 vectors using synthetic oligonucleotides. The DNA is first cloned into a novel M13 vector (based upon M13mp18 or M13mp19), which carries a genetic marker that can be selected against, such as an EcoK or EcoB site, or an amber m...
Enregistré dans:
| Auteurs principaux: | , , |
|---|---|
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
1985
|
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC321798/ https://ncbi.nlm.nih.gov/pubmed/2989795 |
| Tags: |
Ajouter un tag
Pas de tags, Soyez le premier à ajouter un tag!
|