Improved oligonucleotide site-directed mutagenesis using M13 vectors.
An improved method is described for the construction of mutations in M13 vectors using synthetic oligonucleotides. The DNA is first cloned into a novel M13 vector (based upon M13mp18 or M13mp19), which carries a genetic marker that can be selected against, such as an EcoK or EcoB site, or an amber m...
Sábháilte in:
| Foilsithe in: | Nucleic Acids Res |
|---|---|
| Príomhchruthaitheoirí: | , , |
| Formáid: | Artigo |
| Teanga: | Inglês |
| Foilsithe / Cruthaithe: |
Oxford University Press
1985
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| Rochtain ar líne: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC321798/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2989795/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/13.12.4431 |
| Clibeanna: |
Níl clibeanna ann, Bí ar an gcéad duine le clib a chur leis an taifead seo!
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