Improved oligonucleotide site-directed mutagenesis using M13 vectors.
An improved method is described for the construction of mutations in M13 vectors using synthetic oligonucleotides. The DNA is first cloned into a novel M13 vector (based upon M13mp18 or M13mp19), which carries a genetic marker that can be selected against, such as an EcoK or EcoB site, or an amber m...
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| Published in: | Nucleic Acids Res |
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| Main Authors: | , , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
Oxford University Press
1985
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| Online Access: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC321798/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2989795/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/13.12.4431 |
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