Modified base compositions at degenerate positions of a mutagenic oligonucleotide enhance randomness in site-saturation mutagenesis.

Site-saturation mutagenesis, using degenerate oligonucleotide primers, is a frequently used method in introducing various mutations in a selected target codon. Oligonucleotides that are synthesized using equimolar concentrations of nucleoside phosphoramidites (dA, dC, dG, dT) in the positions to be...

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Pubblicato in:	Nucleic Acids Res
Autori principali:	Airaksinen, A, Hovi, T
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	Oxford University Press 1998
Soggetti:	Research Article
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC147293/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9421518/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/26.2.576
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