Modified base compositions at degenerate positions of a mutagenic oligonucleotide enhance randomness in site-saturation mutagenesis.

Site-saturation mutagenesis, using degenerate oligonucleotide primers, is a frequently used method in introducing various mutations in a selected target codon. Oligonucleotides that are synthesized using equimolar concentrations of nucleoside phosphoramidites (dA, dC, dG, dT) in the positions to be...

Descripció completa

Dades bibliogràfiques

Publicat a:	Nucleic Acids Res
Autors principals:	Airaksinen, A, Hovi, T
Format:	Artigo
Idioma:	Inglês
Publicat:	Oxford University Press 1998
Matèries:	Research Article
Accés en línia:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC147293/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9421518/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/26.2.576
Etiquetes:	Afegir etiqueta Sense etiquetes, Sigues el primer a etiquetar aquest registre!