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Site-directed mutagenesis of the binding site for ribosomal protein S8 within 16S ribosomal RNA from Escherichia coli.

Twelve specific alterations have been introduced into the binding site for ribosomal protein S8 in Escherichia coli 16S rRNA. Appropriate rDNA segments were first cloned into bacteriophage M13 vectors and subjected to bisulfite and oligonucleotide-directed mutagenesis in vitro. Subsequently, the mut...

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Main Authors: Gregory, R J, Zimmermann, R A
格式: Artigo
語言:Inglês
出版: 1986
在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC311590/
https://ncbi.nlm.nih.gov/pubmed/3016664
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