Modified base compositions at degenerate positions of a mutagenic oligonucleotide enhance randomness in site-saturation mutagenesis.
Site-saturation mutagenesis, using degenerate oligonucleotide primers, is a frequently used method in introducing various mutations in a selected target codon. Oligonucleotides that are synthesized using equimolar concentrations of nucleoside phosphoramidites (dA, dC, dG, dT) in the positions to be...
שמור ב:
| הוצא לאור ב: | Nucleic Acids Res |
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| Principais autores: | , |
| פורמט: | Artigo |
| שפה: | Inglês |
| יצא לאור: |
Oxford University Press
1998
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| נושאים: | |
| גישה מקוונת: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC147293/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9421518/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/26.2.576 |
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