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Efficient method for site-directed mutagenesis in large plasmids without subcloning
Commonly used methods for site-directed DNA mutagenesis require copying the entire target plasmid. These methods allow relatively easy modification of DNA sequences in small plasmids but become less efficient and faithful for large plasmids, necessitating full sequence verification. Introduction of...
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| Опубликовано в: : | PLoS One |
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| Главные авторы: | , , , , , |
| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
Public Library of Science
2017
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5456045/ https://ncbi.nlm.nih.gov/pubmed/28575024 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0177788 |
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