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An efficient one-step site-directed deletion, insertion, single and multiple-site plasmid mutagenesis protocol

BACKGROUND: Mutagenesis plays an essential role in molecular biology and biochemistry. It has also been used in enzymology and protein science to generate proteins which are more tractable for biophysical techniques. The ability to quickly and specifically mutate a residue(s) in protein is important...

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Détails bibliographiques
Auteurs principaux: Liu, Huanting, Naismith, James H
Format: Artigo
Langue:Inglês
Publié: BioMed Central 2008
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC2629768/
https://ncbi.nlm.nih.gov/pubmed/19055817
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-8-91
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