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REPLACR-mutagenesis, a one-step method for site-directed mutagenesis by recombineering

Mutagenesis is an important tool to study gene regulation, model disease-causing mutations and for functional characterisation of proteins. Most of the current methods for mutagenesis involve multiple step procedures. One of the most accurate methods for genetically altering DNA is recombineering, w...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Trehan, Ashutosh, Kiełbus, Michał, Czapinski, Jakub, Stepulak, Andrzej, Huhtaniemi, Ilpo, Rivero-Müller, Adolfo
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4707547/
https://ncbi.nlm.nih.gov/pubmed/26750263
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep19121
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