Efficient method for site-directed mutagenesis in large plasmids without subcloning

Commonly used methods for site-directed DNA mutagenesis require copying the entire target plasmid. These methods allow relatively easy modification of DNA sequences in small plasmids but become less efficient and faithful for large plasmids, necessitating full sequence verification. Introduction of...

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Bibliografske podrobnosti
izdano v:PLoS One
Main Authors: Hallak, Louay K., Berger, Kelly, Kaspar, Rita, Kwilas, Anna R., Montanaro, Federica, Peeples, Mark E.
Format: Artigo
Jezik:Inglês
Izdano: Public Library of Science 2017
Teme:
Research Article
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC5456045/
https://ncbi.nlm.nih.gov/pubmed/28575024
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0177788
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