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Efficient method for site-directed mutagenesis in large plasmids without subcloning
Commonly used methods for site-directed DNA mutagenesis require copying the entire target plasmid. These methods allow relatively easy modification of DNA sequences in small plasmids but become less efficient and faithful for large plasmids, necessitating full sequence verification. Introduction of...
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| Veröffentlicht in: | PLoS One |
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| Hauptverfasser: | , , , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Public Library of Science
2017
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5456045/ https://ncbi.nlm.nih.gov/pubmed/28575024 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0177788 |
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