A simple and efficient method for site-directed mutagenesis with double-stranded plasmid DNA.

A general, simple and efficient method for preparing site-specific mutations in double-stranded plasmid DNA without the need for special plasmids, bacterial strains or reagents is described. Only one synthetic oligonucleotide for each mutation is required, subcloning is unnecessary and a high effici...

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I whakaputaina i:	Nucleic Acids Res
Ngā kaituhi matua:	Lai, D, Zhu, X, Pestka, S
Hōputu:	Artigo
Reo:	Inglês
I whakaputaina:	Oxford University Press 1993
Urunga tuihono:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC309981/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8371973/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/21.17.3977
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