A simple and efficient method for site-directed mutagenesis with double-stranded plasmid DNA.
A general, simple and efficient method for preparing site-specific mutations in double-stranded plasmid DNA without the need for special plasmids, bacterial strains or reagents is described. Only one synthetic oligonucleotide for each mutation is required, subcloning is unnecessary and a high effici...
Gardado en:
| Publicado en: | Nucleic Acids Res |
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| Principais autores: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
Oxford University Press
1993
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| Acceso en liña: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC309981/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8371973/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/21.17.3977 |
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