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Oligonucleotide-directed double-strand break repair in plasmids of Escherichia coli: a method for site-specific mutagenesis.

A DNA double-strand break can be efficiently repaired in Escherichia coli if an oligodeoxyribonucleotide is provided to direct the repair. The oligonucleotide must be at least 20 residues long and have a sequence identical to sequences flanking the break. The phenomenon can be used to introduce defi...

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Bibliografske podrobnosti
Glavni avtor: Mandecki, W
Format: Artigo
Jezik:Inglês
Izdano: 1986
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC386678/
https://ncbi.nlm.nih.gov/pubmed/3532104
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