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Oligonucleotide-directed double-strand break repair in plasmids of Escherichia coli: a method for site-specific mutagenesis.

A DNA double-strand break can be efficiently repaired in Escherichia coli if an oligodeoxyribonucleotide is provided to direct the repair. The oligonucleotide must be at least 20 residues long and have a sequence identical to sequences flanking the break. The phenomenon can be used to introduce defi...

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Na minha lista:
Sonraí Bibleagrafaíochta
Príomhúdar: Mandecki, W
Formáid: Artigo
Teanga:Inglês
Foilsithe: 1986
Ábhair:
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC386678/
https://ncbi.nlm.nih.gov/pubmed/3532104
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