Oligonucleotide-directed double-strand break repair in plasmids of Escherichia coli: a method for site-specific mutagenesis.
A DNA double-strand break can be efficiently repaired in Escherichia coli if an oligodeoxyribonucleotide is provided to direct the repair. The oligonucleotide must be at least 20 residues long and have a sequence identical to sequences flanking the break. The phenomenon can be used to introduce defi...
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| Publicado en: | Proc Natl Acad Sci U S A |
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| Autor principal: | |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
National Academy of Sciences
1986
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC386678/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3532104/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.83.19.7177 |
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