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A simple and efficient method for site-directed mutagenesis with double-stranded plasmid DNA.
A general, simple and efficient method for preparing site-specific mutations in double-stranded plasmid DNA without the need for special plasmids, bacterial strains or reagents is described. Only one synthetic oligonucleotide for each mutation is required, subcloning is unnecessary and a high effici...
Sparad:
| Huvudupphovsmän: | , , |
|---|---|
| Materialtyp: | Artigo |
| Språk: | Inglês |
| Publicerad: |
1993
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| Länkar: | https://ncbi.nlm.nih.gov/pmc/articles/PMC309981/ https://ncbi.nlm.nih.gov/pubmed/8371973 |
| Taggar: |
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