Efficient method for site-directed mutagenesis in large plasmids without subcloning

Commonly used methods for site-directed DNA mutagenesis require copying the entire target plasmid. These methods allow relatively easy modification of DNA sequences in small plasmids but become less efficient and faithful for large plasmids, necessitating full sequence verification. Introduction of...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:PLoS One
Prif Awduron: Hallak, Louay K., Berger, Kelly, Kaspar, Rita, Kwilas, Anna R., Montanaro, Federica, Peeples, Mark E.
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: Public Library of Science 2017
Pynciau:
Research Article
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC5456045/
https://ncbi.nlm.nih.gov/pubmed/28575024
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0177788
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