A CRISPR/Cas9-based method and primer design tool for seamless genome editing in fission yeast

In the fission yeast Schizosaccharomyces pombe the prevailing approach for gene manipulations is based on homologous recombination of a PCR product that contains genomic target sequences and a selectable marker. The CRISPR/Cas9 system has recently been implemented in fission yeast, which allows for...

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Bibliografische gegevens
Gepubliceerd in:Wellcome Open Res
Hoofdauteurs: Rodríguez-López, María, Cotobal, Cristina, Fernández-Sánchez, Oscar, Borbarán Bravo, Natalia, Oktriani, Risky, Abendroth, Heike, Uka, Dardan, Hoti, Mimoza, Wang, Jin, Zaratiegui, Mikel, Bähler, Jürg
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: F1000Research 2017
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Method Article
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5445975/
https://ncbi.nlm.nih.gov/pubmed/28612052
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.12688/wellcomeopenres.10038.3
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