A CRISPR/Cas9-based method and primer design tool for seamless genome editing in fission yeast

In the fission yeast Schizosaccharomyces pombe the prevailing approach for gene manipulations is based on homologous recombination of a PCR product that contains genomic target sequences and a selectable marker. The CRISPR/Cas9 system has recently been implemented in fission yeast, which allows for...

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Dettagli Bibliografici
Pubblicato in:Wellcome Open Res
Autori principali: Rodríguez-López, María, Cotobal, Cristina, Fernández-Sánchez, Oscar, Borbarán Bravo, Natalia, Oktriani, Risky, Abendroth, Heike, Uka, Dardan, Hoti, Mimoza, Wang, Jin, Zaratiegui, Mikel, Bähler, Jürg
Natura: Artigo
Lingua:Inglês
Pubblicazione: F1000Research 2017
Soggetti:
Method Article
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC5445975/
https://ncbi.nlm.nih.gov/pubmed/28612052
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.12688/wellcomeopenres.10038.3
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