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A CRISPR/Cas9-based method and primer design tool for seamless genome editing in fission yeast
In the fission yeast Schizosaccharomyces pombe the prevailing approach for gene manipulations is based on homologous recombination of a PCR product that contains genomic target sequences and a selectable marker. The CRISPR/Cas9 system has recently been implemented in fission yeast, which allows for...
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| Veröffentlicht in: | Wellcome Open Res |
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| Hauptverfasser: | , , , , , , , , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
F1000Research
2017
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5445975/ https://ncbi.nlm.nih.gov/pubmed/28612052 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.12688/wellcomeopenres.10038.3 |
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