A CRISPR/Cas9-based method and primer design tool for seamless genome editing in fission yeast

In the fission yeast Schizosaccharomyces pombe the prevailing approach for gene manipulations is based on homologous recombination of a PCR product that contains genomic target sequences and a selectable marker. The CRISPR/Cas9 system has recently been implemented in fission yeast, which allows for...

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Udgivet i:Wellcome Open Res
Main Authors: Rodríguez-López, María, Cotobal, Cristina, Fernández-Sánchez, Oscar, Borbarán Bravo, Natalia, Oktriani, Risky, Abendroth, Heike, Uka, Dardan, Hoti, Mimoza, Wang, Jin, Zaratiegui, Mikel, Bähler, Jürg
Format: Artigo
Sprog:Inglês
Udgivet: F1000Research 2017
Fag:
Method Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5445975/
https://ncbi.nlm.nih.gov/pubmed/28612052
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.12688/wellcomeopenres.10038.3
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