A CRISPR/Cas9-based method and primer design tool for seamless genome editing in fission yeast

In the fission yeast Schizosaccharomyces pombe the prevailing approach for gene manipulations is based on homologous recombination of a PCR product that contains genomic target sequences and a selectable marker. The CRISPR/Cas9 system has recently been implemented in fission yeast, which allows for...

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Detalles Bibliográficos
Publicado en:Wellcome Open Res
Autores principales: Rodríguez-López, María, Cotobal, Cristina, Fernández-Sánchez, Oscar, Borbarán Bravo, Natalia, Oktriani, Risky, Abendroth, Heike, Uka, Dardan, Hoti, Mimoza, Wang, Jin, Zaratiegui, Mikel, Bähler, Jürg
Formato: Artigo
Lenguaje:Inglês
Publicado: F1000Research 2017
Materias:
Method Article
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC5445975/
https://ncbi.nlm.nih.gov/pubmed/28612052
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.12688/wellcomeopenres.10038.3
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