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Simplified primer design for PCR-based gene targeting and microarray primer database: two web tools for fission yeast

PCR-based gene targeting is a popular method for manipulating yeast genes in their normal chromosomal locations. The manual design of primers, however, can be cumbersome and error-prone. We have developed a straightforward web-based tool that applies user-specified inputs to automate and simplify th...

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Hlavní autoři: Penkett, Christopher J, Birtle, Zoë E, Bähler, Jürg
Médium: Artigo
Jazyk:Inglês
Vydáno: John Wiley & Sons, Ltd. 2006
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2964512/
https://ncbi.nlm.nih.gov/pubmed/17072893
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/yea.1422
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