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Seamless site-directed mutagenesis of the Saccharomyces cerevisiae genome using CRISPR-Cas9
CRISPR assisted homology directed repair enables the introduction of virtually any modification to the Saccharomyces cerevisiae genome. Of obvious interest is the marker-free and seamless introduction of point mutations. To fulfill this promise, a strategy that effects single nucleotide changes whil...
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| Published in: | J Biol Eng |
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| Main Authors: | , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
BioMed Central
2016
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4850645/ https://ncbi.nlm.nih.gov/pubmed/27134651 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13036-016-0028-1 |
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