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New Vectors for Simple and Streamlined CRISPR-Cas9 Genome Editing in Saccharomyces cerevisiae
CRISPR-Cas9 technology is an important tool for genome editing because the Cas9 endonuclease can induce targeted DNA double-strand breaks. Targeting of the DNA break is typically controlled by a single-guide RNA (sgRNA) – a chimeric RNA containing a structural segment important for Cas9 binding and...
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| Publicado en: | Yeast |
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| Autores principales: | , , , , , , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
2015
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4715497/ https://ncbi.nlm.nih.gov/pubmed/26305040 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/yea.3098 |
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