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Simple CRISPR-Cas9 Genome Editing in S. cerevisiae
CRISPR-Cas9 has emerged as a powerful method for editing the genome in a wide variety of species, since it can generate a specific DNA break when targeted by the Cas9-bound guide RNA. In yeast, Cas9-targeted DNA breaks are used to promote homologous recombination with a mutagenic template DNA, in or...
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| Publicado no: | Curr Protoc Mol Biol |
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| Main Authors: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2019
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6986324/ https://ncbi.nlm.nih.gov/pubmed/31763795 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cpmb.110 |
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