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R-loop formation by dCas9 is mutagenic in Saccharomyces cerevisiae

Cas9 binds and cleaves specific DNA sequences by inducing the formation of an R-loop between the guide RNA and its genomic target. While targeting of active Cas9 to a genomic locus is highly mutagenic because Cas9 creates DNA double strand breaks, targeting of dead Cas9 (dCas9) is presumed not to be...

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Detalhes bibliográficos
Publicado no:Nucleic Acids Res
Main Authors: Laughery, Marian F, Mayes, Hannah C, Pedroza, Ivan K, Wyrick, John J
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2019
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6411842/
https://ncbi.nlm.nih.gov/pubmed/30590793
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gky1278
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