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R-loop formation by dCas9 is mutagenic in Saccharomyces cerevisiae
Cas9 binds and cleaves specific DNA sequences by inducing the formation of an R-loop between the guide RNA and its genomic target. While targeting of active Cas9 to a genomic locus is highly mutagenic because Cas9 creates DNA double strand breaks, targeting of dead Cas9 (dCas9) is presumed not to be...
Enregistré dans:
| Publié dans: | Nucleic Acids Res |
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| Auteurs principaux: | , , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
Oxford University Press
2019
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6411842/ https://ncbi.nlm.nih.gov/pubmed/30590793 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gky1278 |
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