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Efficient multiplexed gene regulation in Saccharomyces cerevisiae using dCas12a
CRISPR Cas12a is an RNA-programmable endonuclease particularly suitable for gene regulation. This is due to its preference for T-rich PAMs that allows it to more easily target AT-rich promoter sequences, and built-in RNase activity which can process a single CRISPR RNA array encoding multiple spacer...
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| Vydáno v: | Nucleic Acids Res |
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| Hlavní autoři: | , , , |
| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
Oxford University Press
2021
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC8287914/ https://ncbi.nlm.nih.gov/pubmed/34197613 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkab529 |
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