A Cas9-based toolkit to program gene expression in Saccharomyces cerevisiae

Despite the extensive use of Saccharomyces cerevisiae as a platform for synthetic biology, strain engineering remains slow and laborious. Here, we employ CRISPR/Cas9 technology to build a cloning-free toolkit that addresses commonly encountered obstacles in metabolic engineering, including chromosom...

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Detalhes bibliográficos
Publicado no:Nucleic Acids Res
Main Authors: Reider Apel, Amanda, d'Espaux, Leo, Wehrs, Maren, Sachs, Daniel, Li, Rachel A., Tong, Gary J., Garber, Megan, Nnadi, Oge, Zhuang, William, Hillson, Nathan J., Keasling, Jay D., Mukhopadhyay, Aindrila
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2017
Assuntos:
Synthetic Biology and Bioengineering
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5224472/
https://ncbi.nlm.nih.gov/pubmed/27899650
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkw1023
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