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Programmable DNA looping using engineered bivalent dCas9 complexes

DNA looping is a ubiquitous and critical feature of gene regulation. Although DNA looping can be efficiently detected, tools to readily manipulate DNA looping are limited. Here we develop CRISPR-based DNA looping reagents for creation of programmable DNA loops. Cleavage-defective Cas9 proteins of di...

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Bibliographic Details
Published in:Nat Commun
Main Authors: Hao, Nan, Shearwin, Keith E., Dodd, Ian B.
Format: Artigo
Language:Inglês
Published: Nature Publishing Group UK 2017
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC5696343/
https://ncbi.nlm.nih.gov/pubmed/29158476
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41467-017-01873-x
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