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Programmable DNA looping using engineered bivalent dCas9 complexes
DNA looping is a ubiquitous and critical feature of gene regulation. Although DNA looping can be efficiently detected, tools to readily manipulate DNA looping are limited. Here we develop CRISPR-based DNA looping reagents for creation of programmable DNA loops. Cleavage-defective Cas9 proteins of di...
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| Publicado no: | Nat Commun |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Nature Publishing Group UK
2017
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5696343/ https://ncbi.nlm.nih.gov/pubmed/29158476 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41467-017-01873-x |
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